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Stem Cell Research; Gene Signatures in Adult and Cord Blood-Derived Mast Cells are Different
Drug Week
15 July 2005
2005 JUL 15 - (NewsRx.com) -- Gene signatures in adult and cord blood-derived mast cells are different.
"In order to better understand the mechanisms governing the display of mast cell characteristics in human mast cells (MCs), such as cord blood (CB)-derived cultured mast cells, peripheral blood (PB)-derived cultured MCs, and differentiated adult-lung cultured MCs, we examined the transcriptomes of these three types MCs using oligonucleotide microarray (GeneChip) and hierarchical clustering analysis," researchers in Japan report.
"The expression profile of CB-derived MCs substantially differed from those of PB-and lung-derived MCs," noted N. Inomata and colleagues, RIKEN.
"In CB-derived MCs, we identified 132 up-regulated transcripts, such as MARCKS, KRT1, TIMP2, SERPINA1, and TLR2, and 428 down-regulated transcripts, such as LTBP3, CDC42BPA, DDO, DICER1, and FCER1A. Moreover, using reverse transcription-polymerase chain reaction (RT-PCR) and flow-activated cell sorter (FACS) analysis, we confirmed the expression of TLR2, which plays an important role in innate immunity, in CB-derived MCs but not in PB-derived MCs."
"In addition, it was observed that CB-derived MCs uniquely release histamine and CCL1, which are produced by human MCs but not by human monocytes, in response to peptidoglycan (PGN), although it had been a controversial issue whether CB-derived MCs could, in fact, induce degranulation in response to PGN."
"These results indicated that in innate immunity MCs derived from neonatal hemopoietic cells might have unique functions compared to their adult counterparts because of different gene profiles," scientists suggested.
Inomata and colleagues published their study in Immunology Letters (Differential gene expression profile between cord blood progenitor-derived and adult progenitor-derived human mast cells. Immunol Lett, 2005;98(2):265-271).
For additional information, contact N. Inomata, RIKEN, Research Center Allergy & Immunology, Research Unit Allergy Transcriptome, Tsurumi Ku, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan.
Publisher contact information for the journal Immunology Letters is: Elsevier Science BV, PO Box 211, 1000 AE Amsterdam, Netherlands.
Keywords: Kanagawa, Japan, Genetics, Genomics, Hematology, Stem Cell Research.
This article was prepared by Drug Week editors from staff and other reports. Copyright 2005, Drug Week via NewsRx.com.
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